Endogenous and Exogenous Fluorescence Platform

platform
Endogenous FLIm and exogenous GFP fluorescence system schematic and representative FLIm and GFP fluorescence intensity images of a locally recellularized antigen-removed bovine pericardium scaffold. Adapted from Alfonso-Garcia, A. et al. Opt Lett 44, 3350 (2019)

Endogenous and exogenous fluorescence emission from biological samples encodes complementary information. Endogenous fluorescence allows studying tissues with minimum disturbance and the absence of contrast agents makes it well suited for clinical translation, whereas the selective use of exogenous labels provides highly specific contrast of features of interest in biological tissue. We design and build instruments that can access both modalities for a variety of applications including vascular tissue engineering.

 

Funding

NIH (National Institutes of Health): R01HL121068, R03EB025565

CIRM (California Institute for Regenerative Medicine): RT3-07879

 

Publications
C. Li, A. Alfonso-Garcia, J. McMasters, J. Bec, B. Weyers, L. Uyesaka, L. Griffiths, A. Panitch, and L. Marcu, "Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor," Methods Appl Fluoresc 7, 044003 (2019).
A. Alfonso-Garcia, C. Li, J. Bec, D. Yankelevich, L. Marcu, and B. Sherlock, "Fiber-based platform for synchronous imaging of endogenous and exogenous fluorescence of biological tissue," Opt Lett 44, 3350-3353 (2019)